Metrohm 940 Professional IC Vario ONE/SeS/PP/Prep 2 Uživatelský manuál

940 Professional IC Vario940 Professional IC Vario ONE/SeS/PP/Prep 2Manual8.940.8020EN

5.12 Suppressor￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭90￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)5.12.3.2 Regenerating the suppression rotorIf

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭91￭ In the software, use the Step com

5.12 Suppressor￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭92￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520) 2Dismantling the suppressor￭ Unscrew union nut

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭93￭ Insert the rotor (25-3) into the

5.12 Suppressor￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭94￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)Replacing parts of the suppressor 1Disconnectin

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭95￭ The rotor's sealing surface

5.13 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭96￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)5.13 Peristaltic pump5.13.1 Notes on op

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭975.13.2.2 Replacing the filterThe f

5.14 Servicing the Metrohm CO2 Suppressor (MCS)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭98￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)5.14 Servicing

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭99 2Regenerating the adsorber materia

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭11 Introduction1.1 Instrument descriptionThe 940

5.17 Separation column￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭100￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)The time upon stopping the watch corres

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭101representative) or on the Internet

5.18 Quality Management and qualification with Metrohm￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭102￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)5.18 Q

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6 Troubleshooting940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭1036 TroubleshootingProblem Cause RemedyMarked

6.1 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭104￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)Problem Cause RemedyNote: 6.2821.180 pump tubing connecti

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6 Troubleshooting940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭105Problem Cause RemedyPeristaltic pump – Pumpt

6.1 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭106￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)Problem Cause Remedy￭ Deaerate the high-pressure pump (se

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭7 Technical specifications940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭1077 Technical specifications7.1 Ref

7.4 Housing￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭108￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)7.4 HousingDimensionsWidth 365 mmHeight 642 mmDep

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭7 Technical specifications940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭1097.7 Eluent degasserMaterial Fluoro

1.1 Instrument description￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)Leak sensorThe leak sensor detects le

7.9 Injection valve￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭110￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)Gradient capabil-ityIsocratic or gradient

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭7 Technical specifications940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭1117.12 Metrohm CO2 Suppressor (MCS)M

7.16 Power connection￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭112￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)7.16 Power connectionRequired voltage 1

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭7 Technical specifications940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭113ETL Listed Mark:￭ Control number 31

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭114￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)8 Warranty (guarantee)Metrohm guarantees that the deliveries

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8 Warranty (guarantee)940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭115The same warranty periods that are spec

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭116￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)9 AccessoriesUp-to-date information on the scope of delivery

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭9 Accessories940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭117The Partslists webpage will be displayed. 7Selec

Index￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭118￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)IndexNumbers/Symbols6-port valve ...

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Index940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭119Pump tubing ... 38Sample degasser ...

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭3Peristaltic pumpThe peristaltic pump is used for

Index￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭120￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)WWarranty ... 114

1.3 Safety instructions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭4￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)The present instrument is suitable for p

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭5Mains voltageWARNINGAn incorrect mains voltage ca

1.4 Symbols and conventions￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭6￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)1.3.5 Recycling and disposalThis pr

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭1 Introduction940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭7NOTEThis symbol highlights additional information

2.1 Front￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭8￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)2 Overview of the instrument2.1 FrontFigure 1 Front1

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Overview of the instrument940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭95Suppressor6Column thermostatWith t

2.2 Rear￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭10￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)2.2 RearFigure 2 Rear1Bottle holderOffers space for t

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Overview of the instrument940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭115Vacuum connectionFor connecting a

2.3 Feed-throughs for capillaries and cables￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭12￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)Openings on the do

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭2 Overview of the instrument940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭13Openings on the back panelFigure 4

2.3 Feed-throughs for capillaries and cables￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭14￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)Figure 5 Ducts for

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭153 Installation3.1 Setting up the instrument3.1

3.2 Capillary connections in the IC system￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭16￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)Also see: PEEK press

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭17￭ The capillary ends do not have exactly flat ed

3.3 Removing the handle￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭18￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)3.3 Removing the handleThe instrument

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭193.4 Removing transport locking screwsTo avoid d

Metrohm AGCH-9100 HerisauSwitzerlandPhone +41 71 353 85 85Fax +41 71 353 89 [email protected] Professional IC Vario940 Professional

3.5 Connecting the drainage tubing and leak sensor￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭20￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)Store the tr

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭21Connecting the drainage tubing 1Cut a piece of s

3.6 Column thermostat￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭22￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)Route the loose end into a waste containe

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭233.7 Connecting the eluent bottleThe eluent is a

3.7 Connecting the eluent bottle￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭24￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520) 2Mounting the tubing adapter

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭25 4Mounting the adsorber tubeNOTEDepending on the

3.8 Connecting the eluent degasser￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭26￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)3.8 Connecting the eluent d

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭27Figure 9 High-pressure pump with purge valve1Pum

3.11 Installing the pulsation absorber￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭28￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)Figure 10 Inline filter1

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭293.12 Injection valveThe injection valve connect

TeachwareMetrohm AGCH-9100 [email protected] documentation is protected by copyright. All rights reserved.Although all the information

3.12 Injection valve￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭30￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)Figure 12 Exchanging the sample loop1Press

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭313.13 SuppressorThe suppressor drive of the 940

3.13 Suppressor￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭32￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)CAUTIONThe rotor may be destroyed during start-

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭33NOTEThe rotor's sealing surface is approxim

3.13 Suppressor￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭34￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭ Insert the rotor into the adapter so that the

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭35Recommended installationAlternative installation

3.13 Suppressor￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭36￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)3.13.2.1 Installing the eluent pathThe eluent

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭37 1Prepare a tubing cartridge of the peristaltic

3.14 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭38￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)Connecting the rinsing solution inlet to

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭39Order num-berName Material Inner diame-terUse6.1

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Table of contents940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭IIITable of contents1 Introduction 11.1 Instr

3.14 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭40￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)Pump tubing Adapter6.1826.390 (yellow/yel

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭41Installing the pump tubingFor this step you need

3.14 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭42￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭ Install the pump tubing connection with

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭43Input Tighten the aspiration tubing (6.1803.020)

3.14 Peristaltic pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭44￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)Setting the contact pressure correctly 1￭

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭45Figure 14 Peristaltic pump1Contact pressure leve

3.15 Metrohm CO2 Suppressor (MCS)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭46￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)ymer membrane. At the same ti

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭47 3Connection to the detectorConnect the detector

3.15 Metrohm CO2 Suppressor (MCS)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭48￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520) 1Preparing the adsorption ca

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭493.16 Installing the detectorThe 940 Professiona

Table of contents￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭IV￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)3.13.2 Connecting the suppressor ...

3.17 Connecting the sample degasser (optional)￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭50￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)Connecting the s

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭513.18 Installing the 6-port valveDuring sample p

3.19 Connecting the instrument to a computer￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭52￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)3.19 Connecting t

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭533.20 Connecting the instrument to the power sup

3.21 Initial start-up￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭54￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)After being switched on, the LED on the f

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭55MagIC Net detects the instrument and all of its

3.22 Connecting and rinsing the guard column￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭56￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520) 5Setting the cont

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭57NOTEThe guard column may not be connected until

3.23 Connecting the separation column￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭58￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭ Optional: In case the g

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭59CAUTIONNew separation columns are filled with so

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Table of contents940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭V5.12.2 Taking care of the suppressor housing .

3.23 Connecting the separation column￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭60￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)Connecting the separation

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭61 2Installing the input of the separation columnT

3.24 Conditioning￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭62￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)3.24 ConditioningAfter installation, after e

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭3 Installation940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭63￭ Visually inspect whether all capillaries and t

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭64￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)4 OperationThe 940 Professional IC Vario – ONE/SeS/PP/Prep 2

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭655 Operation and maintenance5.1 IC

5.1 IC system￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭66￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)5.1.4 Shutting down and starting back upIf the i

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭675.2 Capillary connectionsAll conne

5.4 Column thermostat – Replacing the capillaries￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭68￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)13211112Figur

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭69Replacing the preheating capillarie

Table of contents￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭VI￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)7.19 Electromagnetic compatibility (EMC) ...

5.6 Servicing the eluent degasser￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭70￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)The composition of the eluent

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭71Connecting the connecting tubing 1￭

5.8 Servicing the high-pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭72￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)5.8 Servicing the high-pr

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭73CAUTIONMaintenance work on the high

5.8 Servicing the high-pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭74￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)Cleaning the outlet valve

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭75NOTEThe maximum treatment time in a

5.8 Servicing the high-pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭76￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)Spare parts If the inlet v

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭77NOTEThe maximum treatment time in a

5.8 Servicing the high-pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭78￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭ Has the pressure dissipa

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭79Figure 19 High-pressure pump – cros

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭Table of figures940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭VIITable of figuresFigure 1 Front ...

5.8 Servicing the high-pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭80￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)21Figure 20 Tool for pisto

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭81 3Removing the old piston sealCAUTI

5.8 Servicing the high-pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭82￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)Optional: Cleaning/replaci

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭83Figure 23 Components of the piston

5.8 Servicing the high-pressure pump￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭84￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)You can find these parts i

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭85Connecting the input for the high-p

5.9 Servicing the inline filter￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭86￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)Removing the filterFigure 24 In

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭87Inserting a new filter 1Inserting a

5.10 Servicing the pulsation absorber￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭88￭￭￭￭￭￭￭￭940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)5.10 Servicing the pulsa

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭5 Operation and maintenance940 Professional IC Vario ONE/SeS/PP/Prep 2 (2.940.1520)￭￭￭￭￭￭￭￭89CAUTIONThe suppressor has to be reg